Mar 26, 2009
PEGylation diminishes PAMAM-induced cytotoxicity
Due to their unique structure, PAMAM dendrimers have been used widely in medical applications. However, PAMAM dendrimers bearing amino terminals can be cytotoxic. To over improve the material's biocompatibility, scientists have conjugated PEG to the dendrimers and are now investigating the mechanisms behind the corresponding decrease in cytotoxicity.
As described recently in Nanotechnology, researchers at Huazhong University of Science and Technology, China, have modified dendrimers with PEG of two different molecular weights and different number of chains. The results indicate that conjugation with PEG could effectively reduce the cell apoptosis by attenuating ROS production and inhibiting MMP collapse. Meanwhile, dendrimers conjugated with more PEG of higher molecular weight were seen to be much less cytotoxic.
This study provides an insight into the effects of PEGylation on cytotoxicity at molecular level. The strategy employed considerably functionalized dendrimers and the researchers have extended their work to investigate the effect of PEGylated dendrimers on haemocompatibility. A series of encouraging results will be reported soon.
About the author
Wei Wang is a PhD student in X Yang group at College of Life Science and Technology, Huazhong University of Science and Technology, China. She is currently exploring the toxicity of nanoscale drug delivery systems.