The team, which includes scientists from Southeast University, forms a single nanopore at the molecular scale by pulling a glass tube in a two-step process. The principle of the fabrication is based on the physical footprint of the phase change of paraffin sealed inside a glass tube to create a nanocavity in the broken terminal during the second step of the pulling process.

The glass nanopore fabrication process is simple and requires no special or expensive equipment. Furthermore, the electrical noise of the nanopore is low. The noise of the current was about 1.6 pA, which is comparable to the noise level of 1.2–11.8 pA in a α-hemolysin nanopore.


The stable structure of the nanopore and the low level of electrical noise are ideal for electrically addressing single-molecule blocked events. IgG molecule translocation is detected with low electrical noise. The researchers found that the salt concentration affects the pulse signal modes as biomolecules pass through the device.

A full description of the method and images of glass nanopores made using the technique can be found in the journal Nanotechnology.