A new paper in the journal Nanotechnology demonstrates how a multiparameter analysis of each spike in the signal train can be used to call all 5 bases (A, T, C, G and the epigentically modified 5-methyl-C) with >90% accuracy. Thus true single-molecule chemical analysis is now enabled by a simple tunnel-junction device.

The study shows some short reads of a DNA sequence, which are then used to analyse binding kinetics in a recognition tunneling junction.

Full details can be found in the journal Nanotechnology.