The working procedure of GNOME laser transfection consists of the incubation of cells with spherical AuNPs for 3 hours. The particles sediment on the cell monolayer and attach non-specifically to the cellular membrane. The molecules of interest are added to the culture medium. In the last step, the sample is irradiated by a weakly focused scanning laser beam. The spatial highly localized AuNP-laser interaction provides transient membrane permeabilization via heat generation. In order to facilitate the applicability of GNOME laser transfection for routine usage, an automated and encased set-up is constructed.

Clear transfection time

Using GNOME laser transfection, a green fluorescent protein (GFP) is injected into ZMTH3 cells. Furthermore, the selective induction of apoptosis via Caspase 3 delivery is demonstrated. Compared with other protein transfection techniques, GNOME laser transfection has a well defined time of transfection point, which allows temporal investigations of the protein signaling and trafficking kinetics.

More information can be found in the journal Nanotechnology 25 245101.

Further reading

Labelling of human transferrin by fluorescent gold nanoclusters (June 2011)
Positive or negative? Nanoparticle surface charge affects cell membrane interactions (June 2013)